Journal: Oncology Letters

Article Title: Aberrant expression of hSef and Sprouty4 in endometrial adenocarcinoma

doi: 10.3892/ol.2015.3835

Figure Lengend Snippet: hSef expression in endometrial cancer. Increased hSef expression was observed in endometrial adenocarcinoma, while decreased hSef expression was observed in the vascular endothelial cells of endometrial adenocarcinoma. Immunohistochemical analysis of the expression of hSef in (A-C) endometrial adenocarcinoma and (D and E) normal endometrial tissues, observed at a magnification of ×200. Expression of hSef in (F-H) endometrial carcinoma and (I and J) normal endometrial tissue, observed at a magnification of ×400. Expression of hSef in the blood vessels of normal endometrial tissues at a magnification of (a and b) ×200 and (c) ×400, and in the blood vessels of endometrial adenocarcinoma tissues at a magnification of (d and e) ×200 and (f) ×400 was also determined using immunohistochemical analysis. Each experiment was performed in duplicate. hSef, human similar expression to fibroblast growth factors.

Article Snippet: Subsequent to blocking, the sections were incubated overnight with goat anti-human hSef primary polyclonal antibody diluted in phosphate-buffered saline (PBS; dilution, 1:100; cat no. AF2275; R&D Systems, Inc., Minneapolis, MN, USA) or rabbit anti-human Sprouty4 primary monoclonal antibody diluted in PBS (dilution, 1:100; cat no. ab103114; Abcam, Cambridge, UK) overnight in a wet chamber at 4°C.

Techniques: Expressing, Immunohistochemical staining

Journal: Oncology Letters

Article Title: Aberrant expression of hSef and Sprouty4 in endometrial adenocarcinoma

doi: 10.3892/ol.2015.3835

Figure Lengend Snippet: Immunoscore of hSef expression in endometrial cancer tissue. Increased hSef expression was identified in endometrial adenocarcinoma. (A) Detailed data and (B) mean ± SD of the immunoscore for hSef expression in normal endometrial tissue in the proliferative and secretory phases. (C) Detailed data and (D) mean ± SD of the immunoscore for hSef expression in endometrial adenocarcinoma tissue in the surgical-pathological stage. Each experiment was performed in duplicate. SD, standard deviation; hSef, human similar expression to fibroblast growth factors.

Article Snippet: Subsequent to blocking, the sections were incubated overnight with goat anti-human hSef primary polyclonal antibody diluted in phosphate-buffered saline (PBS; dilution, 1:100; cat no. AF2275; R&D Systems, Inc., Minneapolis, MN, USA) or rabbit anti-human Sprouty4 primary monoclonal antibody diluted in PBS (dilution, 1:100; cat no. ab103114; Abcam, Cambridge, UK) overnight in a wet chamber at 4°C.

Techniques: Expressing, Standard Deviation

Journal: Oncology Letters

Article Title: Aberrant expression of hSef and Sprouty4 in endometrial adenocarcinoma

doi: 10.3892/ol.2015.3835

Figure Lengend Snippet: Decreased hSef expression was observed in the vascular endothelial cells of endometrial cancer tissue. (A) Detailed data and (B) mean ± SD of the immunoscore for hSef expressionin the blood vessels of normal endometrial tissue in the proliferative and secretory phases. (C) Detailed data and (D) mean ± SD of the immunoscore for hSef expression in the blood vessels of endometrial adenocarcinoma tissue in the surgical-pathological stage. Each experiment was performed in duplicate. hSef, human similar expression to fibroblast growth factors; SD, standard deviation.

Article Snippet: Subsequent to blocking, the sections were incubated overnight with goat anti-human hSef primary polyclonal antibody diluted in phosphate-buffered saline (PBS; dilution, 1:100; cat no. AF2275; R&D Systems, Inc., Minneapolis, MN, USA) or rabbit anti-human Sprouty4 primary monoclonal antibody diluted in PBS (dilution, 1:100; cat no. ab103114; Abcam, Cambridge, UK) overnight in a wet chamber at 4°C.

Techniques: Expressing, Standard Deviation